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KMID : 0545120090190050431
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 5 p.431 ~ p.438
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Role of Alkaline Serine Protease, Asp, in Vibrio alginolyticus Virulence and Regulation of Its Expression by LuxO-LuxR Regulatory System
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Rui Hao-Peng
Liu Qin
Wang Qi-Yao
Ma Yue
Liu Huan
Shi Cun-Bin
Zhang Yuan-Xing
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Abstract
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The alkaline serine protease asp, which was shown to be a virulence factor of Vibrio alginolyticus as a purified protein, was cloned from V. alginolyticus EPGS, a strain recently isolated from moribund Epinephelus coioides in an outbreak of vibriosis in a mariculture farm of Shenzhen. The asp null mutant was constructed by homologous recombination with suicide plasmid pNQ705-1. Compared with the wild-type strain, the asp null mutant exhibited a significant decrease of total extracellular protease activity, and caused a 15-fold decrease in virulence of V. alginolyticus. In our previous study, the luxO and luxRval genes from V. alginolyticus MVP01 were cloned and identified, and the luxO-luxRval regulatory couple was shown to regulate various genes expression, suggesting that it played a central role in the quorum sensing system of V. alginolyticus. In this study, the regulation of the asp gene was analyzed by using RT-PCR and quantitative real-time PCR methods; we proved that its transcription was greatly induced at the late large stage of growth and was regulated by luxO-luxRval regulatory system.
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KEYWORD
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Alkaline serine protease, asp, quorum sensing, Vibrio alginolyticus
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